S5395
Superoxide Dismutase from bovine erythrocytes
BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell culture
Sinonimo/i:
SOD, Superoxide: superoxide oxidoreductase
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About This Item
Prodotti consigliati
Origine biologica
bovine erythrocytes
Livello qualitativo
Nome Commerciale
BioReagent
Forma fisica
lyophilized powder
Attività specifica
≥3,000 units/mg protein
PM
32.5 kDa
Confezionamento
pkg of 15000 units
tecniche
cell culture | mammalian: suitable
pH
7.6-10.5
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
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Descrizione generale
Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.
Applicazioni
Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
- for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
- for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
- as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis
Azioni biochim/fisiol
Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
Definizione di unità
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Risultati analitici
For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Protocolli
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
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