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Key Documents

R1003

Sigma-Aldrich

Ribonuclease T1 from Aspergillus oryzae

ammonium sulfate suspension, 300,000-600,000 units/mg protein

Sinonimo/i:

Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Aspergillus sp. (Aspergillus oryzae)

Livello qualitativo

Forma fisica

ammonium sulfate suspension

Attività specifica

300,000-600,000 units/mg protein

PM

11068 by amino acid sequence

tecniche

cell based assay: suitable

Compatibilità

suitable for separating native or denatured proteins, or nucleic acids

applicazioni

cell analysis

Temperatura di conservazione

2-8°C

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Applicazioni

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Azioni biochim/fisiol

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Definizione di unità

One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

Stato fisico

Suspension in 2.8 M (NH4)2SO4 solution

Risultati analitici

Protein determined by E1%/280

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves


Certificati d'analisi (COA)

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Articoli

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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