70856

RNase A Solution

Convenient solution for selective degradation of RNA

• Sinonimo/i: RNase A Enzyme
• Codice UNSPSC: 12352202
• NACRES: NA.54

Proprietà

• Forma fisica: solution
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Concentrazione: 10 mg/mL
• tecniche: DNA purification: suitable
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 10-30°C

Descrizione

Descrizione generale

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Applicazioni

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
• to treat control adult and embryonic thymi cells during staining
• to remove RNA from affibody–mRNA fusion clones before clone screening

Azioni biochim/fisiol

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

Attenzione

Toxicity: Standard Handling (A)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2