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G3776

Sigma-Aldrich

Guanosine 5′-triphosphate sodium salt solution

HPLC purified, aqueous solution for RNA polymerase transcription

Sinonimo/i:

GTP

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About This Item

Numero CAS:
36051-31-7
Numero MDL:
MFCD00077781
Codice UNSPSC:
41106305
ID PubChem:
24895138
NACRES:
NA.52

Origine biologica

Porcine brain
bacterial (Corynebacterium)
yeast

Livello qualitativo

300

Saggio

≥95% (HPLC)

Stato

liquid

Concentrazione

100 mM in H2O (adjusted to pH 7)

Colore

colorless

Attività estranea

DNase, RNase and Nickase, none detected

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Stringa SMILE

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
KZRMTEVIDYXWQW-CYCLDIHTSA-K

Informazioni sul gene

human ... HRAS(3265)

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Categorie correlate

Applicazioni

Guanosine 5′-triphosphate sodium salt solution has been used:

  • for reconstituting transducin during purification of transducin.
  • as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes
  • as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates
  • in DNA-dependent RNA polymerase transcription

Azioni biochim/fisiol

Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion. It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis, and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCJ3892
SLCB3545
SLBW7401
SLBP8495V
SLBL7597V

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In vitro biochemical assays to monitor rhodopsin function.
Sammons J and Gross AK
Methods in Molecular Biology, 884, 167-181 (2012)
Giselle Cerchiaro et al.
Redox report : communications in free radical research, 14(2), 82-92 (2009-04-25)
Levels of oxidized guanosine base in DNA have become a hallmark biomarker in assessing oxidative stress implicated in a variety of disease and toxin-induced states. However, there is evidence that the guanosine in the nucleotide triphosphate pool (GTP) is more
Jonathan A Winger et al.
Biochemistry, 44(10), 4083-4090 (2005-03-09)
The catalytic domains (alpha(cat) and beta(cat)) of alpha1beta1 soluble guanylate cyclase (sGC) were expressed in Escherichia coli and purified to homogeneity. alpha(cat), beta(cat), and the alpha(cat)beta(cat) heterodimeric complex were characterized by analytical gel filtration and circular dichroism spectroscopy, and activity
P Barbier et al.
Biochemistry, 37(2), 758-768 (1998-02-21)
NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active
S A Cohn et al.
The Journal of biological chemistry, 264(8), 4290-4297 (1989-03-15)
We have analyzed the effects of various substrates and inhibitors on the rates of microtubule (MT) motility induced by sea urchin egg kinesin using real-time computer analysis and video-enhanced light microscopy. In the presence of magnesium, 10 mM concentrations of
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