G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-Green Fluorescent Protein
• Numero MDL: MFCD05665217
• Codice UNSPSC: 12352203
• NACRES: NA.56

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates; western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Specificità

Specific for GFP fusion proteins.

Immunogeno

synthetic peptide corresponding amino acids 3-17 of GFP.

Applicazioni

Anti-GFP, N-terminal antibody produced in rabbit has been used:

• in immunoblotting
• in the histochemistry and immunohistochemistry
• in the immunoprecipitation

Azioni biochim/fisiol

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)