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CS0170

Sigma-Aldrich

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Sinonimo/i:

Thioredoxin Reductase Activity Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Livello qualitativo

200

impiego

 kit sufficient for 100 assays (1 mL)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)

Categorie correlate

Descrizione generale

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Applicazioni

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Caratteristiche e vantaggi

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Solo come componenti del kit

N° Catalogo
Descrizione
  • Assay Buffer 5x for Thioredoxin Reductase 30 mL
  • Thioredoxin Reductase 10 μg
  • Thioredoxin Reductase Inhibitor Solution .05 mL
  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
  • NADPH 25 mg
  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Pittogrammi

Health hazard
GHS08

Avvertenze

Warning

Indicazioni di pericolo

H361

Classi di pericolo

Repr. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F

Punto d’infiammabilità (°C)

87 °C

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