T0910

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

• Numero CAS: 52500-60-4
• Numero MDL: MFCD00132423
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: Escherichia coli
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: essentially salt-free, lyophilized powder
• Attività specifica: ≥3 units/mg protein
• PM: 11.7 kDa
• tecniche: activity assay: suitable
• Solubilità: water: 1.90-2.10 mg/mL, clear, colorless
• N° accesso ID proteina: M26133
• N° accesso UniProt: P0AA25
• applicazioni: cell analysis
• Temperatura di conservazione: −20°C
• Informazioni sul gene: Escherichia coli K12 ... trxA(948289)

Descrizione

Descrizione generale

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Applicazioni

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Azioni biochim/fisiol

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.

Mediates the reduction of disulfide bonds in proteins.

Altre note

GenBank^® Accession Number M26133.

Definizione di unità

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Note legali

GenBank is a registered trademark of United States Department of Health and Human Services

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)