598101

Thioredoxin, Human, Recombinant, E. coli

• Sinonimo/i: Thioredoxin, Human, Recombinant, E. coli, rhTRX
• Numero CAS: 52500-60-4
• Numero MDL: MFCD00132423
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 100
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: lyophilized solid
• Attività specifica: ≥1.7 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.

Altre note

Noda, N., et al. 1997. Nippon Rinsho 55, 461.
Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.
Masutani, H., et al. 1996. Immunol. Lett. 54, 67.
Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.
Saito, N., et al. 1995. J. Immunol. 154, 3194.
Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable