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Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)
Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç
RESUMO

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

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Sigma-Aldrich
Fosfato de sódio, ACS reagent, 98.0-102.0%
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D-(+)-Glicose, ≥99.5% (GC)
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D-(+)-Glicose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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Ureia, powder, BioReagent, for molecular biology, suitable for cell culture
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Ácido L-ascórbico, powder, suitable for cell culture, γ-irradiated
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Ácido L-ascórbico, BioXtra, ≥99.0%, crystalline
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Dextrose, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
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Fosfato de sódio monobásico, 98-100.5% (calc. to the dried substance), meets analytical specification of Ph. Eur., BP, USP, FCC, E 339
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D-(+)-Glicose, ≥99.5% (GC), BioXtra
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Ácido L-ascórbico, suitable for cell culture, suitable for plant cell culture, ≥98%
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Urea solution, BioUltra, ~8 M in H2O
Supelco
Urea, 8 M (after reconstitution with 16 mL high purity water)
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Ácido úrico, ≥99%, crystalline
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Fosfato de sódio monobásico, BioUltra, for molecular biology, ≥99.0% (T)
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Ácido L-ascórbico, reagent grade, crystalline
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Nickel(II) oxide, 99.99% trace metals basis
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D-(+)-Glicose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
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Ácido L-ascórbico, analytical standard
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Ácido L-ascórbico, reagent grade
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Ureia, BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)
Supelco
D-(+)-Glicose, analytical standard
Sigma-Aldrich
Ureia, BioUltra, for molecular biology, 99% (T)
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Ácido L-ascórbico, meets USP testing specifications
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Ácido úrico, BioXtra, ≥99% (HPLC)
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Ureia, suitable for electrophoresis
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Fosfato de sódio, ≥99.99% trace metals basis
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Ureia, ACS reagent, 99.0-100.5%
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Ácido L-ascórbico, FCC, FG
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Nickel(II) oxide, green, −325 mesh, 99%
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Ácido L-ascórbico, 99%