G004845

GC Analysis of Resveratrol in Red Wine (Spiked) on SLB^®-5ms after SPME using a 85 μm Polyacrylate Fiber

suitable for GC, application for SPME

Propriedades

• técnica(s): gas chromatography (GC): suitable; solid phase microextraction (SPME): suitable
• parâmetros de teste: sample/matrix: 3 mL of red wine (California merlot) spiked with resveratrol at 100 μg/L then diluted 3:1 in 12% ethanol:water; SPME fiber: 85 μm polyacrylate (57304, 57305, 57294-U); extraction: immersion for 15 min at room temperature while stirring at 400 rpm, fiber gently blotted with a Kimwipe to remove excess water, (then derivatization by immersion in a 4 mL vial containing 5 μL of Sylon-BFT (fresh vial for each analysis than was allowed to equilabrate 60-90 minutes prior to use) for 20 min); desorption process: 280 °C for 2 min; column: SLB-5ms; 30 m x 0.25 mm I.D., 0.25 μm (28471-U); oven: 100 °C (1 min), 10 °C/min to 325 °C (3 min); inj. temp.: 280 °C; detector: GC/MS; MSD interface: 325 °C; scan range: m/z 40-450; carrier gas: helium, 1 mL/min, constant; liner: 0.75 mm I.D., SPME; sample: Red wine
• adequação: application for SPME
• aplicação(ões): food and beverages

Descrição

Nota de análise

In this study, the extraction and analysis of resveratrol from red wine is demonstrated using solid phase microextraction (SPME) and GC/MS. The presence of three hydroxyl (OH) groups makes it necessary to derivatize this compound prior to GC analysis. Derivatization was conducted, after extraction, directly on the SPME fiber by exposing it to the vapors of a silyating reagent. The derivative of resveratrol was then analyzed directly from the fiber using GC/MS. The polyacrylate fiber, which is suitable for the extraction of polar semivolatiles and is more resistant to swelling than other types of fibers was used in the analysis. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.

Informações legais

SLB is a registered trademark of Merck KGaA, Darmstadt, Germany