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Documentos Principais

TOX8

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Resazurin based

Sinônimo(s):

Fluorescent resazurin toxicology assay

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About This Item

Número CE:
Código UNSPSC:
12352207
NACRES:
NA.84

Formulário

liquid

Nível de qualidade

uso

1 kit sufficient for 2,000 tests

embalagem

pkg of 1 kit

condição de armazenamento

dry at room temperature

técnica(s)

protein quantification: suitable

aplicação(ões)

cell analysis
detection

método de detecção

colorimetric
fluorometric

temperatura de armazenamento

2-8°C

Descrição geral

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Aplicação

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts
  • to determine the cell proliferation rate in murine pre-osteoblasts
  • to determine the metabolic activity of microfluidic and monolayer cultures

Ações bioquímicas/fisiológicas

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
José Manuel Quesada-Gómez et al.
The Journal of steroid biochemistry and molecular biology, 148, 214-218 (2014-12-03)
The nitrogen-containing or nitrogenous bisphosphonates (N-BF) are currently the main class of drugs used for the treatment of diseases characterized by an increased bone resorption. Preliminary data suggest that N-BF have also direct or indirect anti-tumoral effects, and recent evidence
A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Maria Elisa Giuliani et al.
Toxicology in vitro : an international journal published in association with BIBRA, 61, 104603-104603 (2019-07-23)
The precision-cut tissue slices (PCTS) represent a largely used biological model in mammalian research. This ex vivo approach offers the main advantages of in vitro systems, while maintaining the natural architecture of the tissue. The use of PCTS in toxicological

Artigos

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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