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TOX4

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Neutral Red based

Sinônimo(s):

NR uptake assay, Neutral red assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.84

Formulário

liquid

Nível de qualidade

uso

 kit sufficient for 1,000 tests

embalagem

pkg of 1 kit

condição de armazenamento

dry at room temperature

λmax

540 nm

aplicação(ões)

cell analysis
detection

método de detecção

colorimetric

temperatura de armazenamento

2-8°C

Descrição geral

For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.

Aplicação

In Vitro Toxicology Assay Kit, Neutral Red based has been used:
  • to measure the lysosomal enzyme activity in HB8902 cells
  • to study the cell viability of human alveolar epithelial cells
  • to study the cell viability in fibroblasts

Ações bioquímicas/fisiológicas

Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

73.4 °F

Ponto de fulgor (°C)

23 °C


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