TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Sinônimo(s):
NR uptake assay, Neutral red assay
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About This Item
Produtos recomendados
Formulário
liquid
Nível de qualidade
uso
kit sufficient for 1,000 tests
embalagem
pkg of 1 kit
condição de armazenamento
dry at room temperature
λmax
540 nm
aplicação(ões)
cell analysis
detection
método de detecção
colorimetric
temperatura de armazenamento
2-8°C
Descrição geral
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Aplicação
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Ações bioquímicas/fisiológicas
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Código de classe de armazenamento
3 - Flammable liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
73.4 °F
Ponto de fulgor (°C)
23 °C
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In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
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