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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Sinônimo(s):

mitochondrial activity assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.84

uso

 kit sufficient for 1,000 tests

Nível de qualidade

200

embalagem

pkg of 1 kit

condição de armazenamento

dry at room temperature

λmax

570 nm

aplicação(ões)

cell analysis
detection

método de detecção

colorimetric

temperatura de armazenamento

2-8°C

Descrição geral

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Aplicação

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Ações bioquímicas/fisiológicas

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

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Classificações de perigo

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Órgãos-alvo

Central nervous system, Respiratory system

Código de classe de armazenamento

3 - Flammable liquids

Ponto de fulgor (°F)

53.6 °F

Ponto de fulgor (°C)

12 °C

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Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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