06432379001

Cell Viability Imaging Kit 5x96

• Código UNSPSC: 12352200
• NACRES: NA.55

Propriedades

• uso: sufficient for 5 96-well plate(s)
• Nível de qualidade: 100
• embalagem: pkg of 1 kit
• fabricante/nome comercial: Roche
• condição de armazenamento: protect from light
• fluorescência: λ_ex 361 nm; λ_em 486 nm (nuclei dye); λ_ex 490 nm; λ_em 515 nm (viable dye); λ_ex 535 nm; λ_em 617 nm (dead dye)
• método de detecção: fluorometric
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C (−15°C to −25°C)

Descrição

Descrição geral

The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.

The kit is designed for 5 × 96 reactions for use in medium to high throughput analysis of cell behavior.

• Determine total cell number using fluorescence staining of cell nuclei.
• Fluorimetrically determine the percentage of dead cells based on membrane permeability.
• Verify the labeling of viable cells quantified by fluorescence staining using a vital dye

Aplicação

Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.

Características e benefícios

Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:

• No risk of losing cells in the one step assay: There are no fixation or washing steps.
• Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
• Obtain highly reproducible results using automated imaging and data evaluation with Roche′s Cellavista System.

Embalagem

1 kit containing 3 components.

Nota de preparo

Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.

Armazenamento e estabilidade

Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)

Outras notas

For life science research only. Not for use in diagnostic procedures.

Somente componentes do kit

• Nuclei Dye (blue cap) for fluorimetric determination of cell count based on labeling of cell nuclei
• Dead Dye (red cap) for fluorimetric determination of dead cells based on cell permeability
• Viable Dye ( green cap) for fluorimetric determination of viable cells based on metabolic activity

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Warning
• Frases de perigo: H341
• Declarações de precaução: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classificações de perigo: Muta. 2
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable