T3573

RANK Ligand/TRANCE human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Sinônimo(s): Osteoclast Differentiation Factor (ODF), Osteoprotegerin Ligand (OPGL), Receptor Activator of NF-KB Ligand (RANKL), TNF-related activation-induced cytokines (TRANCE)
• Número CAS: 207621-35-0
• Número MDL: MFCD01867142
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• recombinante: expressed in NSO cells
• Ensaio: >90% (SDS-PAGE)
• forma: lyophilized powder
• potência: 1.5-7.5 ng/mL ED₅₀
• peso molecular: glycolysylated recombinant product ~35 kDa by SDS-PAGE; calculated mol wt 23 kDa
• embalagem: pkg of 10 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• Impurezas: endotoxin, tested
• nº de adesão UniProt: O14788
• aplicação(ões): cell analysis
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... TNFSF11(8600)

Descrição

Descrição geral

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.

Aplicação

RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation.

RANK Ligand/TRANCE human has been used to stimulate differentiation.

Ações bioquímicas/fisiológicas

RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity.

RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.

forma física

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.

Nota de análise

The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)