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Documentos Principais

SRP2108

Sigma-Aldrich

p53 (1-81), mutant, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sinônimo(s):

FLJ92943, LFS1, P53, TRP53

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.26

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥80% (SDS-PAGE)

Formulário

frozen liquid

peso molecular

~34.5 kDa

embalagem

pkg of 10 μg

concentração

450 μg/mL

cor

clear colorless

nº de adesão NCBI

NM_000546

nº de adesão UniProt

P04637

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... TP53(7157)

Descrição geral

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1.

Ações bioquímicas/fisiológicas

The protein has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein.
p53 was identified as a tumor suppressor by showing that this protein has the ability to block transformation and to inhibit tumor cell growth. In addition, p53 is a transcription factor capable of regulating the expression of a subset of downstream genes. Mutation of two specific N-terminal residues in p53 (residues Leu22 and Trp23) impairs the ability of p53 to transactivate and has been correlated with its ability to bind TAFII40 and TAFII60 (or TAFII31 and TAFII70) (7, 8) suggesting that one or both of these interactions is important for activation. Mutation of residues 22 and 23 to Ala does not affect binding to TBP, although mutation of these residues to charged amino acids has been reported to disrupt the p53-TBP interaction. Different mutations in p53 gene have been characterized in a variety of human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis.

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
032201037

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Cooper GM.
The Cell: A Molecular Approach null
S J Baker et al.
Science (New York, N.Y.), 249(4971), 912-915 (1990-08-24)
Mutations of the p53 gene occur commonly in colorectal carcinomas and the wild-type p53 allele is often concomitantly deleted. These findings suggest that the wild-type gene may act as a suppressor of colorectal carcinoma cell growth. To test this hypothesis
C A Finlay et al.
Cell, 57(7), 1083-1093 (1989-06-30)
DNA clones of the wild-type p53 proto-oncogene inhibit the ability of E1A plus ras or mutant p53 plus ras-activated oncogenes to transform primary rat embryo fibroblasts. The rare clones of transformed foci that result from E1A plus ras plus wild-type
D Michalovitz et al.
Cell, 62(4), 671-680 (1990-08-24)
Mutant p53 can contribute to transformation, while wild-type (wt) p53 is not oncogenic and actually inhibits transformation. Furthermore, wt p53 may act as a suppressor gene in human carcinogenesis. We now describe the temperature-sensitive behavior of a particular mutant, p53val135.
Mutations in the p53 Tumor Suppressor Gene
Noa Rivlin
Genes & Cancer, 2(4), 466-474 (2011)
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