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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

Sinônimo(s):

Super Optimal broth

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grau

for molecular biology

esterilidade

0.2 μm filtered

Formulário

liquid

composição

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

técnica(s)

microbiological culture: suitable

aplicação(ões)

agriculture

temperatura de armazenamento

2-8°C

adequação

nonselective for Escherichia coli
nonselective for coliforms

Categorias relacionadas

Descrição geral

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Aplicação

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

Características e benefícios

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SLCF7416
SLCG7766
SLBW4174
SLCD1893
SLBZ9713

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Meg L Flanagan et al.
Methods in molecular biology (Clifton, N.J.), 378, 33-52 (2008-07-09)
As a source of recombinant antigen, soluble constant fragment (Fc) fusion proteins have become valuable reagents for immunotherapy and laboratory investigations. Additional applications for these reagents include flow cytometry, immunohistochemistry, and in vitro activity assays. To aid investigators in the
Kenneth Smith et al.
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General protocols for growth of competent cells and their transformation (uptake of DNA).

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Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

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