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B9146

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%, powder, suitable for identification of lac+ bacterial colonies

Sinônimo(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Fórmula empírica (Notação de Hill):
C14H15BrClNO6
Número CAS:
7240-90-6
Peso molecular:
408.63
Beilstein:
1402009
Número CE:
230-640-8
Número MDL:
MFCD00005666
Código UNSPSC:
12352204
ID de substância PubChem:
57653968
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

Nível de qualidade

200

Ensaio

≥98%

forma

powder

solubilidade

DMF: methanol (1:1): soluble 50 mg/mL

adequação

suitable for identification of lac+ bacterial colonies

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

chave InChI

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Aplicação

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Reconstituição

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

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Nº do produto
Descrição
Preços

94433

β-Galactosidase stain

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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