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Key Documents

CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Sinônimo(s):

Competent E. coli cells

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About This Item

Código UNSPSC:
41106202
NACRES:
NA.85

product name

BL21(DE3) Chemically Competent Cells, for protein expression

fonte biológica

Escherichia coli

grau

for molecular biology

modo de crescimento

adherent or suspension

morfologia

rod shaped

técnica(s)

microbiological culture: suitable

transformação celular

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Descrição geral

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Aplicação

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Características e benefícios

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Componentes

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

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Código de classe de armazenamento

10 - Combustible liquids

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Conteúdo relacionado

BL21 Chemically Competent Cells

BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.

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