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L2897

Sigma-Aldrich

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Sinônimo(s):

Lennox broth, Luria Bertani Agar

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

grau

for molecular biology

Nível de qualidade

200

esterilidade

non-sterile

forma

powder

composição

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

técnica(s)

microbiological culture: suitable

aplicação(ões)

food and beverages
microbiology

temperatura de armazenamento

room temp

adequação

nonselective for Escherichia coli
nonselective for coliforms

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Descrição geral

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Aplicação

LB Broth with agar (Lennox) has been used for:

  • the transformation of competent E. coli cells
  • to cultivate Ecc-15 bacteria
  • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
  • transmission electron microscopy (TEM) and immunohistochemistry (IHC)
It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Características e benefícios

Lennox LB powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Easy scale-up using larger package sizes
  • Standard formulation

Nota de preparo

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Informações legais

Zeocin is a registered trademark of Cayla Sarl

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Protocolos

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

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