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L7533

Sigma-Aldrich

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

Sinônimo(s):

LB broth

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grau

for molecular biology

Nível de qualidade

200

esterilidade

non-sterile

forma

powder

composição

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

técnica(s)

microbiological culture: suitable

aplicação(ões)

agriculture
food and beverages
microbiology

temperatura de armazenamento

room temp

adequação

nonselective for Escherichia coli
nonselective for coliforms

Categorias relacionadas

Descrição geral

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Aplicação

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Características e benefícios

Lennox EZMix powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Nota de preparo

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Reconstituição

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Informações legais

Zeocin is a registered trademark of Cayla Sarl

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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