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52062

Millipore

LB-Agar, high salt

suitable for microbiology, NutriSelect® Plus

Sinônimo(s):

Luria Bertani Agar, high salt

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About This Item

Código UNSPSC:
41171605
NACRES:
NA.85

esterilidade

non-sterile

Nível de qualidade

forma

powder

prazo de validade

limited shelf life, expiry date on the label

composição

agar, 15 g/L
casein enzymic hydrolysate, 10 g/L
sodium chloride, 10 g/L
yeast extract, 5 g/L

fabricante/nome comercial

NutriSelect® Plus

técnica(s)

microbiological culture: suitable

pH final

7.5±0.2 (25 °C)

aplicação(ões)

clinical testing
food and beverages
life science and biopharma

microbiology

adequação

nonselective for Escherichia coli
nonselective for bacteria (General Media)
nonselective for coliforms

Descrição geral

LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7.

Aplicação

LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.
Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.

Nota de preparo

Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Escherichia coli Physiology in Luria-Bertani Broth.
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746?8749-8746?8749 (2007)
Rapid and simple determination of theEscherichia coli phylogenetic group.
Clermont O, et al.
Applied and Environmental Microbiology, 66(10), 4555-4558 (2000)
Commercial Lysogeny Broth culture media and oxidative stress: a cautious tale.
Ezraty B, et al.
Free Radical Biology & Medicine, 74, 245-251 (2014)

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