RECOMT
Thrombin CleanCleave™ Kit
Sinônimo(s):
Thrombin Cleavage Kit
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.56
Produtos recomendados
Formulário
suspension
Nível de qualidade
peso molecular
37 kDa
técnica(s)
protein purification: suitable
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Aplicação
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Características e benefícios
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Quantidade
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Informações legais
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Somente componentes do kit
Nº do produto
Descrição
- Thrombin Cleavage Buffer, 10× 10 mL
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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