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P5994

Sigma-Aldrich

Substrato fosfatase

40 mg tablets

Sinônimo(s):

4-Nitrofenil fosfato, pNPP

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About This Item

Fórmula linear:
O2NC6H4OP(O)(ONa)2 · 6H2O
Número CAS:
333338-18-4
Peso molecular:
371.14
Número CE:
224-246-5
Número MDL:
MFCD00066288
Código UNSPSC:
12352204
ID de substância PubChem:
24898723
NACRES:
NA.54

Nível de qualidade

200

forma

tablet

concentração

36-44 mg/tablet

Impurezas

p-nitrophenol, essentially free

solubilidade

H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

chave InChI

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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Descrição geral

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Aplicação

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Substrato cromogênico para uso com procedimentos de ELISA com fosfatases ácidas e alcalinas. O substrato gera um produto final solúvel de cor amarela que pode ser lido por espectrofotometria em 405 nm. A reação de pNPP pode ser interrompida com NaOH 3 N e lida em 405 nm.

Quantidade

Contains 40 mg substrate per tablet

Nota de preparo

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.

Outras notas

Substrato para determinação de fosfatases alcalinas e ácidas.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Gemma Mestres et al.
Acta biomaterialia, 8(3), 1169-1179 (2011-12-14)
α-Tricalcium phosphate (α-TCP) is widely used as a reactant in calcium phosphate cements. This work aims at doping α-TCP with silicon with a twofold objective. On the one hand, to study the effect of Si addition on the stability and
Elucidating human phosphatase-substrate networks
Li X, et al.
Science Signaling, 6(275), rs10-rs10 (2013)
Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength
Henriksen K, et al.
PLoS ONE, 6(11), e27482-e27482 (2011)
Tetsuya Harakuni et al.
Infection and immunity, 73(9), 5654-5665 (2005-08-23)
Noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. Although the conjugation of vaccine antigens with the B subunit of cholera toxin (CTB) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot
Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
Huang D, et al.
International journal of nanomedicine, 12(11), 7483-7483 (2017)
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Artigos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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