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OGS1499

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PSF-CMV-PURO-NH2-ALB - HUMAN ALBUMIN SECRETION PLASMID

plasmid vector for molecular cloning

Sinônimo(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200

forma

buffered aqueous solution

peso molecular

size 6197 bp

seleção de bactérias

kanamycin

seleção de células de mamífero

puromycin

Origem de replicação

pUC (500 copies)

clivagem do peptídeo

no cleavage

localização da etiqueta do peptídeo

N-terminal

Promotor

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

gene repórter

none

Sinal de secreção

human albumin

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Descrição geral

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an Human Albumin (Alb) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Sequência

To view sequence information for this product, please visit the product page

Nota de análise

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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