Pular para o conteúdo
Merck
Todas as fotos(2)

Key Documents

OGS1913

Sigma-Aldrich

PSF-TEF1-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG YEAST PLASMID

plasmid vector for molecular cloning

Sinônimo(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

etiqueta

10-His tagged

forma

buffered aqueous solution

peso molecular

size 6768 bp

seleção de bactérias

kanamycin

Origem de replicação

2Micron
pUC (500 copies)

clivagem do peptídeo

TEV

localização da etiqueta do peptídeo

C-terminal

Promotor

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

gene repórter

none

Condições de expedição

ambient

temperatura de armazenamento

−20°C

seleção de leveduras

uracil

Descrição geral

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Sequência

To view sequence information for this product, please visit the product page

Nota de análise

To view the Certificate of Analysis for this product, please visit www.oxgene.com

produto relacionado

Nº do produto
Descrição
Preços

OGS10

PSF-CMV-KAN - KANAMYCIN RESISTANCE PLASMID, plasmid vector for molecular cloning

OGS103

PSF-CMV-RLUC - CMV RENILLA LUCIFERASE PLASMID, plasmid vector for molecular cloning

OGS1120

PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

OGS1175

PSF-CMV-PURO-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

OGS1658

PSF-TEF1-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG YEAST PLASMID, plasmid vector for molecular cloning

OGS1913

PSF-TEF1-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG YEAST PLASMID, plasmid vector for molecular cloning

OGS2767

PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS2828

PSF-OXB20-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS394

PSF-CMV-PGK-PURO - DUAL PROMOTER PUROMYCIN PLASMID, plasmid vector for molecular cloning

OGS412

PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID, plasmid vector for molecular cloning

OGS502

PSF-LACI - LACI PROMOTER BACTERIAL VECTOR, plasmid vector for molecular cloning

OGS505

PSF-CAG-KAN - CAG PROMOTER VECTOR, plasmid vector for molecular cloning

OGS553

PSF-OXB1 - WEAK STRENGTH BACTERIAL PROMOTER PLASMID, plasmid vector for molecular cloning

OGS588

PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID, plasmid vector for molecular cloning

PP2356

Mammalian Promoter Vector Set, plasmid vectors for molecular cloning

PP2381

Yeast Signal Peptide Vector Set, plasmid vectors for molecular cloning

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

PP2394

Bacterial FLAG® Tag Vector Set, plasmid vectors for molecular cloning

B11001

EnPresso® B, Growth system for expressing protein in bacteria

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

Artigos

SnapFast™ Restriction Site Functions

Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.

Sequencing Primers

A range of forward and reverse sequencing primers that allow you to sequence any insert that you make into a particular position within any plasmid. Where possible, the binding sites for each of these primers is conserved.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica