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OGS2828

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PSF-OXB20-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG BACTERIAL PLASMID

plasmid vector for molecular cloning

Sinônimo(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.85

recombinante

expressed in E. coli

etiqueta

6-His tagged
FLAG® tagged

Formulário

buffered aqueous solution

peso molecular

size 4991 bp

seleção de bactérias

kanamycin

Origem de replicação

pUC (500 copies)

clivagem do peptídeo

EKT

localização da etiqueta do peptídeo

N-terminal

Promotor

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

gene repórter

none

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Descrição geral

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an n-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH. This plasmid also contains a secondary Flag protein tag. The sequence of this tag is: DYKDDDDKWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.

About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Sequência

To view sequence information for this product, please visit the product page

Nota de análise

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Informações legais

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

SnapFast™ Restriction Site Functions

Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.

Sequencing Primers

A range of forward and reverse sequencing primers that allow you to sequence any insert that you make into a particular position within any plasmid. Where possible, the binding sites for each of these primers is conserved.

Global Trade Item Number

SKUGTIN
OGS2828-5UG4061837165634

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