MITOISO1

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

• Sinônimo(s): Mitochondria Purification Kit
• Número CE: 232-650-8
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• Nível de qualidade: 200
• uso: sufficient for 10-20 g (animal tissue); sufficient for 50 assays (2 mL)
• prazo de validade: 2 yr
• técnica(s): fractionation: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Mitochondria Isolation Kit has been used:

• for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
• for the isolation of mitochondria from glomeruli and heart
• for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Características e benefícios

• Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
• Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
• Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
• Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Componentes do kit também disponíveis separadamente

• T9201 Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 10 - Combustible liquids