CS0720
Citrate Synthase Assay Kit
1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)
Sinônimo(s):
Citrate Synthase Activity Test Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.28
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Descrição geral
The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.
Ações bioquímicas/fisiológicas
Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.
Nota de análise
The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.
Somente componentes do kit
Nº do produto
Descrição
- Assay buffer for citrate synthase 5x 25 mL
- Bicine buffer 10 mL
- CelLytic™ M Cell Lysis Reagent 10 mL
- DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg
- Acetyl coenzyme A 25 mg
- Oxaloacetic acid 1 g
- Citrate synthase (positive control) .1 mL
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Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
10 - Combustible liquids
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