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CYTOCOX1

Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

Sinônimo(s):

Cytochrome c Oxidase Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

Nível de qualidade

200

uso

sufficient for 100 tests

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... COX1(4512)

Categorias relacionadas

Aplicação

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Ações bioquímicas/fisiológicas

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Características e benefícios

  • Simple, optimized protocol - Obtain reproducible results without special training needs
  • Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
  • Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
  • May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
  • Use to analyze the intactness of mitochondrial membranes
  • Simple colorimetric measurement of solution

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

Pictogramas

Corrosion
GHS05

Palavra indicadora

Danger

Frases de perigo

H315,H318

Classificações de perigo

Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
079M4190V
11190179
058M4121V
11180208
11170506

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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

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Global Trade Item Number

SKUGTIN
CYTOCOX1-1KT4061833553237

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