GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Produtos recomendados
uso
sufficient for 50 g (tissue)
Nível de qualidade
técnica(s)
fractionation: suitable
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Aplicação
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Nota de análise
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
Componentes do kit também disponíveis separadamente
Nº do produto
Descrição
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
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Artigos
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
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