MAK354

Homocysteine Assay Kit

sufficient for 100 fluorometric tests

• Sinônimo(s): Homocysteine Quantitation Kit
• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• uso: sufficient for 100 fluorometric tests
• método de detecção: fluorometric
• doença(s) relevante(s): cardiovascular diseases

Descrição

Descrição geral

Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine (‘free′ homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke.

Características e benefícios

Compatible with high-throughput handling systems.

Adequação

Suitable for the determination of total homocysteine concentration in plasma or serum.

Princípio

The Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (λex = 658 nm/λem = 708 nm).  The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 μM homocysteine.

Informações de segurança

• Pictogramas: GHS08,GHS05
• Palavra indicadora: Danger
• Frases de perigo: H290,H315,H319,H334,H412
• Declarações de precaução: P234 - P261 - P264 - P273 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable