M9400
β-Mannosidase from Helix pomatia
5-30 units/mL, ammonium sulfate suspension, crude extract
Sinônimo(s):
β-D-Mannoside mannohydrolase
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32
Produtos recomendados
forma
ammonium sulfate suspension
Nível de qualidade
qualidade
crude extract
peso molecular
94 kDa
concentração
5-30 units/mL
atividade externa
β-N-acetylglucosaminidase and α-mannosidase <1.0%
temperatura de armazenamento
2-8°C
Procurando produtos similares? Visita Guia de comparação de produtos
Descrição geral
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
Aplicação
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
Ações bioquímicas/fisiológicas
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
Definição da unidade
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
forma física
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
inibidor
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Umme Afsari Zahura et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 162(1-3), 24-33 (2012-03-01)
A β-D-mannosidase (EC 3.2.1.25) with a molecular mass of approximately 100 kDa was purified from the digestive fluid of a marine gastropod Aplysia kurodai by ammonium sulfate fractionation followed by column chromatographies on TOYOPEARL Butyl-650 M, TOYOPEARL DEAE-650 M, and
Sunghoon Park et al.
Protein and peptide letters, 20(1), 107-114 (2012-07-14)
Sequence alignments and homology modeling of Pyrococcus furiosus thermostable glycosidase (PFTG) showed that the residue 150 is conserved as tryptophan in β-glycosidase and in other related enzymes such as β- mannosidase and β-galactosidase. To elucidate the relationship between the substrate
Barbora Fliedrová et al.
Protein expression and purification, 85(2), 159-164 (2012-08-14)
β-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal β-linked mannoside in various sugar chains. cDNA corresponding to the β-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The β-mannosidase gene
Farage H Al-Ghazzewi et al.
Journal of the science of food and agriculture, 92(11), 2394-2396 (2012-04-13)
Glucomannan polysaccharides may be hydrolysed to lower molecular weight molecules using acids or enzymes, specifically mannanases or cellulases. Mannanases (β-mannanases) hydrolyse β-(1-4)-linked mannose residues randomly in mannans whilst cellulases (β-glucanase) hydrolyse β-(1-4)-linked glucose residues. The molecular weight of the hydrolysate
Cristina Martínez-Andújar et al.
The Plant journal : for cell and molecular biology, 71(4), 575-586 (2012-03-31)
The micropylar region of endosperm in a seed, which is adjacent to the radicle tip, is called the 'endosperm cap', and is specifically activated before radicle emergence. This activation of the endosperm cap is a widespread phenomenon among species and
Artigos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica