M9170

Macrophage Colony-Stimulating Factor from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinônimo(s): mCSF-1, mM-CSF, CSF-1, M-CSF
• Número CAS: 81627-83-0
• Número CE: 214-684-5
• Número MDL: MFCD00166591
• Código UNSPSC: 12352202
• eCl@ss: 32190102
• NACRES: NA.77

Propriedades

• Nome do produto: Macrophage Colony-Stimulating Factor from mouse, M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• fonte biológica: mouse
• Nível de qualidade: 200
• recombinante: expressed in E. coli
• Ensaio: ≥98% (SDS-PAGE)
• Formulário: lyophilized powder
• potência: 0.500-3.000 ng/mL ED₅₀
• qualidade: endotoxin tested
• peso molecular: dimer 18.2 kDa (containing 156 amino acid residues)
• embalagem: pkg of 10 μg
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1.000 EU/μg
• cor: white
• nº de adesão UniProt: P07141
• temperatura de armazenamento: −20°C
• Informações sobre genes: mouse ... Csf1(12977)

Descrição

Aplicação

Macrophage Colony-Stimulating Factor from mouse has been used:

• in the initiation of osteoclastogenesis in bone marrow monocyte cells
• to stimulate osteoclast differentiation in non-adherent hematopoietic cell
• for the generation of blood-derived stem cells

Ações bioquímicas/fisiológicas

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF augments many of the physiological functions of macrophages, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing and glycosylation the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

forma física

Lyophilized from a sterile filtered buffered aqueous solution.

Nota de análise

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319,H335
• Declarações de precaução: P261 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves