L9641
Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)
Sinônimo(s):
LPS
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About This Item
Produtos recomendados
fonte biológica
Escherichia coli (EH100 Ra mutant)
Nível de qualidade
Formulário
lyophilized powder
Impurezas
<3% Protein (Lowry)
cor
white to yellow
solubilidade
water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Descrição geral
This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.
Ações bioquímicas/fisiológicas
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Nota de preparo
Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Outras notas
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 2 Oral
Código de classe de armazenamento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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