L8777
Lectin from Phytolacca americana (pokeweed)
lyophilized powder, BioReagent, suitable for cell culture
Sinônimo(s):
PWM, Pokeweed mitogen
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About This Item
Produtos recomendados
esterilidade
aseptically filled
Nível de qualidade
linha de produto
BioReagent
forma
lyophilized powder
potência
≤0.3 μg/mL mitogenic activity
técnica(s)
cell culture | mammalian: suitable
solubilidade
PBS: 1 mg/mL
temperatura de armazenamento
2-8°C
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Descrição geral
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Aplicação
Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).
Ações bioquímicas/fisiológicas
Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Qualidade
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Nota de análise
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1 - Skin Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificados de análise (COA)
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