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L2755

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O128:B12

purified by phenol extraction

Sinônimo(s):

LPS

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About This Item

Número CE:
Número MDL:
Código UNSPSC:
12352201
NACRES:
NA.25

fonte biológica

Escherichia coli (O128:B12)

Nível de qualidade

Formulário

lyophilized powder

purificado por

phenol extraction

Impurezas

<3% Protein (Lowry)

cor

white to yellow cast

solubilidade

water: soluble

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This product is phenol extracted from E. coli serotype O128:B12. The source strain is CDC 2440-69.

Aplicação

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Ações bioquímicas/fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparo

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Outras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

produto relacionado

Pictogramas

Skull and crossbones

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Acute Tox. 2 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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