L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• Sinônimo(s): Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• Número CAS: 9001-60-9
• Número da licença da enzima: 1.1.1.27 (BRENDA, IUBMB)
• Número CE: 232-617-8
• Número MDL: MFCD00131466
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: bovine heart
• Nível de qualidade: 200
• tipo: Type XVII
• Ensaio: 5.0—15.0 mg protein/mL (biuret)
• forma: buffered aqueous glycerol solution
• atividade específica: ≥400 units/mg protein
• peso molecular: 140 kDa
• fabricante/nome comercial: Sigma-Aldrich
• técnica(s): cell based assay: suitable
• Impurezas: <10 μg/mg protein free ammonium
• cor: white
• pH: 7.5
• traços de cátion: NH₄⁺: ≤10 μg/mg protein
• nº de adesão UniProt: P19858; Q5E9B1
• aplicação(ões): life science and biopharma
• atividade externa: glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%; pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: cow ... LDHA(281274) , LDHB(281275)

Descrição

Descrição geral

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

Aplicação

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

Ações bioquímicas/fisiológicas

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Definição da unidade

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

forma física

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Nota de análise

Protein determined by biuret

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1