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L2500

Sigma-Aldrich

L-Lactic Dehydrogenase from rabbit muscle

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

Sinônimo(s):

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

Número CAS:
9001-60-9
Número da licença da enzima:
1.1.1.27 (BRENDA, IUBMB)
Número CE:
232-617-8
Número MDL:
MFCD00131466
Código UNSPSC:
12352204
NACRES:
NA.54

tipo

Type II

Nível de qualidade

200

Formulário

ammonium sulfate suspension

atividade específica

800-1,200 units/mg protein

peso molecular

140 kDa

atividade externa

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

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Descrição geral

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Aplicação

L-Lactic Dehydrogenase from rabbit muscle has been used:
  • for protein-binding measurement
  • to detect the action of oxaloacetate decarboxylase
  • to determine serum L- and D-lactate

Ações bioquímicas/fisiológicas

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.

Definição da unidade

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

forma física

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Nota de análise

Protein determined by biuret.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCF1086
SLCD5636
SLCB3953
SLBT2408
SLBP7664V

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Visite a Biblioteca de Documentos

Nutritional Biochemistry, 233-233 (1999)
Autofluorescence from NADH conformations associated with different metabolic pathways monitored using nanosecond-gated spectroscopy and spectral phasor analysis
Maltas J, et al.
Analytical Chemistry, 87(10), 5117-5124 (2015)
Anaerobic growth of Escherichia coli on D-tartrate depends on the fumarate carrier DcuB and fumarase, rather than the L-tartrate carrier TtdT and L-tartrate dehydratase
Kim OB, et al.
Archives of Microbiology, 188(6), 583-589 (2007)
José L Vasconcelos-Lima et al.
Genetics and molecular biology, 44(4), e20210127-e20210127 (2021-11-23)
Variations in dissolved oxygen levels are common in the Amazonian aquatic environments and the aquatic organisms that inhabit these environments developed a variety of adaptive responses to deal with such conditions. Some Amazonian fish species are tolerant to low oxygen
Duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Short-chain fatty acid and lactate absorption, saliva production, and blood metabolites
Schwaiger T, et al.
Journal of Animal Science, 91(12), 5743-5753 (2013)
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