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Documentos Principais

GE17-5055-01

HiTrap® DEAE Fast Flow

Cytiva 17-5055-01, pack of 5 × 1 mL

Sinônimo(s):

DEAE resin, Ion-exchange chromatography resin

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

diethylaminoethyl

descrição

Ion Exchanger Type (value)

embalagem

pack of 5 × 1 mL

fabricante/nome comercial

Cytiva 17-5055-01

Parâmetros

42 psi

tamanho do leito

7 mm × 25 mm

volume do leito

1 mL

D.I. da coluna

7 mm

Matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

2-14

Faixa de trabalho

2-12

adequação

suitable for bioprocess medium

Categorias relacionadas

Descrição geral

HiTrap® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Aplicação

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.


The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose 4 Fast FLow (high sub) has a diethylaminopropyl group attached.

Características e benefícios

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Certificados de análise (COA)

Lot/Batch Number

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Protocolos

This page covers the use of Sepharose Fast Flow for purification of proteins.

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Sample Preparation in Ion Exchange Chromatography

Global Trade Item Number

SKUGTIN
GE17-5055-014061837621598

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