GE28-9782-45

HiScreen^™, DEAE Fast Flow

Cytiva 28-9782-45

• Código UNSPSC: 47101511
• NACRES: NA.56

Propriedades

• ligand: diethylaminoethyl
• descrição: Ion Exchanger Type (value)
• Formulário: prepacked column
• reticulação: cross-linked
• embalagem: pkg of 1 ea
• fabricante/nome comercial: Cytiva 28-9782-45
• condição de armazenamento: (20% Ethanol)
• Parâmetros: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• técnica(s): liquid chromatography (LC): suitable
• tamanho do leito: 7.7 mm × 100 mm
• volume do leito: 4.7 mL
• D.I. da coluna: 7.7 mm
• Matriz: 6% cross-linked agarose
• Grupo ativo da matriz: N⁺(C₂H₅)₂H functional group
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 2-14
• Faixa de trabalho: 2-12
• capacidade: binding capacity(~ 110 mg HAS/mL resin)
• adequação: suitable for bioprocess medium
• técnica de separação: anion exchange

Descrição

Descrição geral

HiScreen^™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Aplicação

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen^™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Características e benefícios

• DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose^™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
• Prepacked, ready-to-use columns for convenient process development optimization.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids