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Key Documents

GE17-5156-01

HiTrap® Q Fast Flow

Cytiva 17-5156-01, pack of 5 × 5 mL

Sinônimo(s):

HiTrap Q Column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

quaternary amine

descrição

Ion Exchanger Type (value)

embalagem

pack of 5 × 5 mL

fabricante/nome comercial

Cytiva 17-5156-01

Parâmetros

42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning

2-14

working range

2-12

adequação

suitable for bioprocess medium

Descrição geral

HiTrap® Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Características e benefícios

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to perform a purification of His-tagged membrane proteins with products from GE Healthcare.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Protocolos

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page covers the use of Sepharose Fast Flow for purification of proteins.

Ver tudo

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica