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F8646

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

FITC conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

condição de armazenamento

protect from light

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

Especificidade

Binds mouse IgG; does not bind other mouse Igs.

Imunogênio

Purified mouse IgG

Aplicação

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

Outras notas

Antibody adsorbed with bovine, equine, and human serum proteins.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparo

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves


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F Gay et al.
Journal of immunological methods, 184(1), 15-28 (1995-07-17)
The adhesion of parasitized red blood cells (PRBC) to the endothelium (sequestration) may contribute to the pathogenic events in severe human malaria caused by P. falciparum. However, the factors involved in the pathophysiology, especially cerebral malaria are poorly understood. Previously
Takashi Kanaya et al.
Research in veterinary science, 82(2), 141-149 (2006-09-05)
M cells play a central role in the initiation of mucosal immune responses. However, a primary source of difficulty for investigations of this is the lack of an available specific marker for bovine M cells. As M cells possess irregular
K A Muczynski et al.
Journal of immunology (Baltimore, Md. : 1950), 160(7), 3207-3216 (1998-04-08)
We compared HLA class II expression in a human melanoma line (a nonprofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class II expression in an EBV-transformed B lymphoblastoid cell line (a professional APC) from the
Gabrielle Dias Salton et al.
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Eukaryote initiation factor 2 subunit β (eIF2β) plays a crucial role in regulation protein synthesis, which mediates the interaction of eIF2 with mRNA. eIF2β contains evolutionarily conserved polylysine stretches in amino-terminal region and a zinc finger motif in the carboxy-terminus.
J Gysin et al.
Infection and immunity, 67(12), 6596-6602 (1999-11-24)
We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and

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