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F4143

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
MFCD00162628
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

conjugado

FITC conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

condição de armazenamento

protect from light

técnica(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:128

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

IgG antibody is secreted by B cells and is found in blood and extracellular fluids and
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system.

Especificidade

Binds mouse IgG; does not bind other mouse Igs.

Imunogênio

purified mouse IgG, Fc fragment

Aplicação

Anti-Mouse IgG (Fc specific)-FITC antibody has been used in
  • immunohistochemistry
  • immunofluorescence
  • flow cytometry
  • immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Ações bioquímicas/fisiológicas

IgG provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Outras notas

Antibody adsorbed with bovine, equine and human serum proteins.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparo

Adsorbed to reduce background staining with bovine, horse or human samples.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análise (COA)

Lot/Batch Number
079M4808V
21190209-1
018M4797V
107M4895V
057M4823V

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Here, we enhanced the popular yeast display method by multiple rounds of DNA and protein engineering. We introduced surface exposure-tailored reporters, eUnaG2 and DnbALFA, creating a new platform of C and N terminal fusion vectors. The optimization of eUnaG2 resulted
Qiong Wu et al.
Applied and environmental microbiology, 82(4), 1173-1182 (2015-12-15)
Escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. We explored the effects of the probiotic Lactobacillus rhamnosus GR-1 on ameliorating E. coli-induced inflammation and cell damage in primary bovine
P Xu et al.
Iranian journal of veterinary research, 22(1), 65-71 (2021-06-22)
Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from
Qiong Wu et al.
Frontiers in microbiology, 9, 1661-1661 (2018-08-09)
Escherichia coli is a common cause of mastitis in dairy cows. The adaptor protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) synergizes with caspase-1 to regulate inflammasome activation during pathogen infection. Here, the ASC gene was knocked out
Chiara Bernardini et al.
International journal of molecular sciences, 21(15) (2020-07-30)
Hydrogen sulfide (H2S) is now considered not only for its toxicity, but also as an endogenously produced gas transmitter with multiple physiological roles, also in maintaining and regulating stem cell physiology. In the present work, we evaluated the effect of
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