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CELLYTPN1

Sigma-Aldrich

CelLytic™ PN Isolation/Extraction Kit

For plant leaves

Sinônimo(s):

Cell lysis kit

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

Nível de qualidade

200

uso

 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

técnica(s)

ChIP: suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

CelLytic™-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Aplicação

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

Características e benefícios

Efficient Nuclei Isolation and Protein Extraction: CelLytic™ PN Isolation/Extraction Kit provides a fast and efficient method for isolating nuclei and extracting functional nuclear proteins from plant leaves, saving time and effort for academic researchers.

Versatility: The isolated nuclei can be used as a source for chromatin, genomic DNA, RNA, etc., making CelLytic™ PN Isolation/Extraction Kit a versatile solution for plant molecular biology research.

Suitable for Detection of DNA-Protein Interactions: The nuclear protein extract obtained using this kit is ideal for detecting DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis, western blot assay, and similar techniques, which is crucial for researchers studying transcription factors and chromatin remodeling.

Plant Model Specific Protocol: The CelLytic™ PN Isolation/Extraction Kit offers a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis, making it an easy-to-follow solution for researchers working with these plant models.

Rapid Extraction: The CelLytic™ PN Isolation/Extraction Kit provides academic researchers with a rapid extraction method, allowing them to quickly extract functional nuclear proteins from plant leaves for their research.

Outras notas

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Informações legais

CelLytic is a trademark of Sigma-Aldrich Co. LLC

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Preços

08168

Timestrip Plus™ 8 °C

Palavra indicadora

Danger

Frases de perigo

H302,H315,H318,H410

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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