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Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

forma

dry powder

temperatura de armazenamento

2-8°C

Aplicação

This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
  • Fraction 1: Soluble/Cytoplasmic Proteins
  • Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Características e benefícios

  • Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
  • Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
  • Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
  • Pre-weighed dry blends - Stable and easy to reconstitute
  • Conveniently Packaged - No waste. Use only the amount needed.

Informações legais

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • C0356Protein Extraction Reagent Type 4 23 mLSDS
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS
  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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W M Freeman et al.
Neuroscience, 159(1), 183-195 (2009-01-13)
Declining cognitive performance is associated with increasing age, even in the absence of overt pathological processes. We and others have reported that declining cognitive performance is associated with age-related changes in brain glucose utilization, long-term potentiation and paired-pulse facilitation, protein
Maria De Angelis et al.
Electrophoresis, 31(13), 2126-2136 (2010-07-02)
Food allergy is recognized as one of the major health concerns. It is estimated that ca. 4% of the population is affected by food allergenic disorders. Food allergies are defined as IgE-mediated hypersensitivity reactions. Foods such as peanuts, tree nuts
Diliana Dancheva Simeonova et al.
Molecular & cellular proteomics : MCP, 8(1), 122-131 (2008-08-22)
We present here a new approach that enabled the identification of a new protein from a bacterial strain with unknown genomic background using a combination of inverted PCR with degenerate primers derived from N-terminal protein sequences and high resolution peptide
Susanne K Pedersen et al.
Journal of proteome research, 2(3), 303-311 (2003-06-20)
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and
FcR? Presence in TCR Complex of Double-Negative T Cells Is Critical for Their Regulatory Function.
Thomson, C.W., et al.
Journal of Immunological Methods, 177, 2250-2257 (2006)
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