BRL-1

94121649, rat liver, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: BRL-1, 94121649
• fonte biológica: rat liver
• modo de crescimento: Adherent
• cariótipo: Diploid or pseudodiploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat epithelial cells

Descrição de linhagem celular

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Aplicação

Feeder cells for co-culture with freshly isolated hepatocytes.

Meio de cultura

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Rotina de subcultura

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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