H4-II-E-C3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061112, rat liver, Epithelial

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: rat liver
• embalagem: tube of 5 μg 85061112-DNA-5UG; pkg of vial of cells 85061112-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat liver hepatoma

Descrição de linhagem celular

From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy.

Aplicação

Enzymatic and virus studies: Type C virus

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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