C2-Rev 7

94101907, rat liver, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: C2-Rev 7, 94101907
• fonte biológica: rat liver
• modo de crescimento: Adherent
• cariótipo: Hyperdiploid, 51 chromosomes
• morfologia: Epithelial
• produtos: Albumin and hepatocyte markers
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat hepatoma [HGPRT-]

Descrição de linhagem celular

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

Aplicação

Gene expression studies.

Meio de cultura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

Outras notas

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