H4II

89042702, rat skin, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: H4II, 89042702
• fonte biológica: rat skin
• modo de crescimento: Adherent
• cariótipo: 52 chromosomes
• morfologia: Epithelial
• produtos: Albumin, transferrin, prothrombin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat skin Hepatocellular carcinoma

Descrição de linhagem celular

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

Aplicação

Gene expression analysis.

Meio de cultura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

Outras notas

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